Obsessing about the antialiasing in a still frame viewed at 200% or 400% is a complete waste of your time. In practical terms, the only way to judge the quality of the lines in your video is to watch the video at 100% scale in real time. If you want to increase the quality of the antialiasing, increase the bit depth, but you will not be able to see it unless you have a high bit depth monitor and when the video is compressed to an 8-bit codec for delivery the stair stepping is going to return. Otherwise, there is no such thing as a perfect line. So what's the point? The point is that if you want perfect lines in a video the lines must be horizontal and vertical and perfectly aligned with the pixel grid. So what's the point of that paragraph? The point is that there will always be stair-stepping (aliasing) and the quality of the antialiasing can only be increased by increasing the bit depth but you can't see any increase in quality unless you have a high bit depth monitor. Changing the interpretation rules for aliasing only changes the rate at which the colors change. You will never see the improvement in antialiasing on an 8-bit monitor.
If you have a 10-bit monitor you can see the stair-stepping in the colors change as you increase bit depth in the project, but if you have an 8-bit monitor you are limited to 256 shades. The color of each of the two rows will be divided into sections based on the color depth and available shades as top row get progressively lighter and the bottom row gets progressively darker.
The line will be interpreted and except for the exact middle and each end, the line will be 2 pixels thick. Take a black line one pixel wide on a white background that starts precisely on 10, 10 and ends precisely on 266, 11. If a line does not perfectly line up with the grid it will appear to have stair steps. Video is limited to the pixel grid, there are no vectors in the final product. Other than than you can do what most of the users do if this situation get's annoying and that's to soft the edges with some box blur with lower iteration and lower blur radios and see where that get's you. I see you set the quality of the layer to bicubic, see if bilinear looks better.
the only thing I can recommend for better anti-aliasing is actually don't use continuously rasterize but instead convert to shape which if fact gives smoother antialiasing than CR of Ai layers, and also check the layer's interpret footage for more options -> more accurate on anti-aliasing. take the same shape in illustrator and turn on pixel preview and magnify to 200% and you will see the same antialiasing steps. Other than that, in my opinion the antialiasing you are showing doesn't look anything out of the ordinary. I would check your artboard options, see that its reference point is in whole pixels. Guess we'll have to see for ourselves how nuke handles it.
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